par Nguyen, Anh Dung 
;Duport, Francois ;Bouwens, Arno ;Vanholsbeeck, Frédérique ;Egrise, Dominique ;Van Simaeys, Gaëtan ;Emplit, Philippe ;Goldman, Serge ;Gorza, Simon-Pierre
Référence Optics express, 23, 17, page (22667-22675), 245319
Publication Publié, 2015-08-19
;Duport, Francois ;Bouwens, Arno ;Vanholsbeeck, Frédérique ;Egrise, Dominique ;Van Simaeys, Gaëtan ;Emplit, Philippe ;Goldman, Serge ;Gorza, Simon-Pierre Référence Optics express, 23, 17, page (22667-22675), 245319
Publication Publié, 2015-08-19
Article révisé par les pairs
| Résumé : | We demonstrate a significant resolution enhancement beyondthe conventional limit in multiphoton microscopy (MPM) using saturatedexcitation of fluorescence. Our technique achieves super-resolved imagingby temporally modulating the excitation laser-intensity and demodulatingthe higher harmonics from the saturated fluorescence signal. The improvementof the lateral and axial resolutions is measured on a sample offluorescent microspheres. While the third harmonic already provides anenhanced resolution, we show that a further improvement can be obtainedwith an appropriate linear combination of the demodulated harmonics.Finally, we present in vitro imaging of fluorescent microspheres incorporatedin HeLa cells to show that this technique performs well in biologicalsamples. |
