• Citer

Effects of the diazafluoranthen derivative AC-3579 on rat liver. A - Isolation and characterization of lamellate cytosomes and subcellular localization of the drug

par May, Claude ;Doriaux, Myriam;Thys, Olivier
Référence PASCAL. F 52, Biochimie, biophysique moléculaire, biologie moléculaire et cellulaire, 34, 2-3, page (187-196)
Publication Publié, 1979

Article révisé par les pairs

• Détails
• Contenu

Résumé :

Lamellate cytosomes induced in rat hepatocytes by the diazafluoranthen derivative AC-3579 were isolated and purified by centrifugation of a resuspended 10,000 g pellet on a discontinuous sucrose gradient. Well preserved purified lamellate cytosomes similar to those observed in liver cells in situ were collected at a density between 1.10-1.14. In purified lamellate cytosomes, phospholipid/protein and cholesterol/phospholipid ratio's were comparable to those of the smooth microsomes of treated animals. The pattern of individual phospholipids of the cytosomes was similar to that of the whole homogenate. These results suggest that the cytosomal material corresponds to sequestered smooth endoplasmic reticulum membranes. After administration of labelled AC-3579, the specific radioactivity was selectively increased in fractions containing the lamellate cytosomes. These data favour the hypothesis that AC-3579 induced cytosomes result from the formation of a complex between the drug and the phospholipids. In smooth microsomes of treated rats, the phospholipid/protein ratio was increased and the cholesterol/phospholipid ratio markedly decreased as compared to the controls. This indicates that the drug induces alterations in the biochemical composition of the membranes.