par Vincke, Bernard 
;Devleeschouwer, Michel ;Patriarche, Gaston
Référence Analytical letters, 18, 13 B, page (1593-1606)
Publication Publié, 1985
;Devleeschouwer, Michel ;Patriarche, Gaston Référence Analytical letters, 18, 13 B, page (1593-1606)
Publication Publié, 1985
Article révisé par les pairs
| Résumé : | L-ascorbic acid membrane electrodes based upon the use of four classes of biocatalysts immobilized at an oxygen electrode are evaluated and compared in terms of electrode properties and operating requirements. Isolated ascorbate oxydase enzyme in soluble form and in covalent binding matrices, peel of cucumber and living bacterial cells of Enterobacter agglomerans strain, respectively, are employed as biocatalytic layers. The physico-chemical factors, life times and interferences are discussed in details. The low stability of the soluble enzyme sensor does not allow its analytical utilization, but the immobilized enzyme, bacterial and tissue electrodes can be used, even in multivitamin pharmaceutical formulations. The common linear range of those three biosensors are of 4.10-6 M to 7.10-4 M with a precision and a reproducibility of ± 3 %. |
