par Vanhaelen Fastré, Renée 
;Vanhaelen, Maurice
Référence Journal of chromatography, 179, 1, page (131-142)
Publication Publié, 1979-11
;Vanhaelen, Maurice Référence Journal of chromatography, 179, 1, page (131-142)
Publication Publié, 1979-11
Article révisé par les pairs
| Résumé : | Both partition chromatography on octadecyl bonded phase and adsorption chromatography on silica gel have been used to study (i) the separation of 1a-hydroxyvitamin D3, 25-hydroxyvitamin D3 and 1a,25-dihydroxyvitamin D3, (ii) the separation of these hydroxyvitamins D3 from their corresponding prehydroxyvitamins D3, and (iii) the separation and the quantitative determination of hydroxyvitamins D3 and prehydroxyvitamins D3 in pharmaceutical formulations. The separate determination of hydroxyvitamins D3 and prehydroxyvitamins D3 was found to be possible only on silica gel. Conversion factors for prehydroxyvitamins D3 and hydroxyvitamins D3 were calculated from measurements of UV absorption at 254 nm after thermal isomerization of the hydroxyvitamins D3. © 1979. |
