par Malmendier, Claude 
;Feremans, Walter ;Fontaine, Marie
Référence Artery, 6, 2, page (144-156)
Publication Publié, 1979
;Feremans, Walter ;Fontaine, Marie Référence Artery, 6, 2, page (144-156)
Publication Publié, 1979
Article révisé par les pairs
| Résumé : | All procedures such as incubaton, dialysis, and concentration used in the preparation of desialyzed lipoproteins, reduced the triglyceride and cholesterol concentrations of human very low density lipoproteins (VLDL). The neuraminidase preparation used contained no lipolytic activity but some proteolytic activity. Sialic acid removal on VLDL modified the particle's electrophoretic mobility on agarose and the migration of apoproteins C-III2 and C-III1 to a C-III0 mobility on PAGE. Apo C-III1 appeared more resistant to enzyme action. Sialic acid removal from VLDL also caused the formation of aggregates of 8000 Å as demonstrated by electron microscopy, and as suggested by the increase in turbidity. The above modifications may be responsible for the changes in catabolism of desialyzed lipoproteins reported in animals. This report represents preliminary data leading to subsequent studies on the turnover of desialyzed lipoproteins in man. |
