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Evaluation of a commercial enzyme immunomembrane filter assay for detection of respiratory syncytial virus in clinical specimens

par Van Beers, Danielle ;De Foor, Marc;Di Cesare, Lina;Vandenvelde, Christian
Référence European journal of clinical microbiology & infectious diseases, 10, 12, page (1073-1076)
Publication Publié, 1991

Article révisé par les pairs

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Résumé :

A commercial enzyme immunomembrane filter assay (EIFA) for respiratory syncytial virus (RSV) was compared prospectively with isolation in cell culture and an enzyme immunoassay. A total of 595 respiratory specimens, mostly from pediatric patients, were examined. The EIFA was 70.96 % sensitive and 72.40 % specific in comparison with cell culture. Results for 40 specimens (6.72 %) were uninterpretable, mainly due to filtration difficulties. Twenty-one (25 %) of 84 specimens whose results were initially considered false-positive were subsequently confirmed positive after a blocking test with bovine anti-RSV serum. On the basis of the total number of confirmed positive results, the sensitivity and the specificity of the test were 87.90 % and 75.77 %, respectively.