par Puissant, Françoise 
;Degueldre, Michel ;Buisson, L.;Leroy, Fernand
Référence Gamete research, 13, 3, page (223-230)
Publication Publié, 1986
;Degueldre, Michel ;Buisson, L.;Leroy, Fernand Référence Gamete research, 13, 3, page (223-230)
Publication Publié, 1986
Article révisé par les pairs
| Résumé : | The effects of varying pH levels on superovulated mouse oocytes before in vitro fertilization, culture, and transfer were investigated. Mouse oocytes acidified for 1 hour with 20% CO2(pH 6.9) exhibited a wide range of alterations. Five hours after insemination, 25% of these eggs showed different degrees of cytolysis and their perivitelline space contained numerous spermatozoa. At 12 hours normal-looking eggs from this group showed a higher proportion of polypronuclear zygotes than the control group (exposed, 51%; controls, 18%). Acidification thus markedly increased the permeability of the zona pellucida and/or interfered with the normal block to polyspermy. Fewer eggs achieved the two-cell stage among groups submitted to acidotic pH levels (6.9 to 6.6). In contrast, alkaline conditions (pH 7.8) did not reduce the proportion of two-cell embryos. However, only eggs maintained at pH 7.5 were capable of producing 80% of blastocysts. In other groups developmental blockage occurred mainly between stages 2 and 4. A significant reduction was found in the proportion of recipient females becoming pregnant after intrauterine transfer of blastocysts originating from CO2- treated oocytes. This difference might be related to the existence of a large polyploid population among experimental eggs. |
