par Viré, Jean-Claude ;Patriarche, Gaston ;Gallo-Hermosa, Bl.
Référence Analytica chimica acta, 196, C, page (205-212)
Publication Publié, 1987
Article révisé par les pairs
Résumé : The electrochemical behaviour of midazolam [7-chloro-5-(o-fluorophenyl)-3H-(2′- methyllimidazo) [1,5-a]-benzodiazepine was studied by polarography and cyclic voltammetry. The irreversible two-electron were is not strongly affected by the imidazole ring or the 5-o-fluorophenyl substituent, but the latter increases the rate of the hydrolysis in acidic media. Kinetic parameters are evaluated for midazolam and three of its hydroxylated metabolites. The hydrolysis is a first-order reaction initially but becomes second order. The 3-hydroxy matabolites are more easily hydrolyzed than midazolam. Midazolam (10-4-10-7 M) can be quanitified by using differential-pulse polarography; the detection limit is 6 × 10-8 M. © 1987.