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Adaptation of the primer extension preamplification (PEP) reaction for preimplantation diagnosis: Single blastomere analysis using short PEP protocols

par Sermon, Karen;Lissens, Willy;Joris, Hubert;Van Steirteghem, André;Liebaers, Ingeborg
Référence Molecular human reproduction, 2, 3, page (209-212)
Publication Publié, 1996

Article révisé par les pairs

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Résumé :

Primer extension preamplification (PEP) was first described as a method for whole genome amplification, starting from a single cell, originally a spermatozoon, in order to perform genetic recombination studies. Its usefulness for preimplantation diagnosis was shown soon after; the only drawback was the length of the procedure (>14 h). We have developed a shorter PEP protocol for single human blastomeres, enabling us to examine several genetic loci of interest in human genetic diseases with a good amplification efficiency. © European Society for Human Reproduction and Embryology.