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A general ampC active site oligonucleotide probe for gram-negative rods.

par Willard-Gallo, Karen ;Moody, J A;Peterson, L R
Référence Molecular and cellular probes, 5, 2, page (97-102)
Publication Publié, 1991-04

Article révisé par les pairs

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Résumé :

We have developed a 33 mer probe that hybridizes to the serine active site of the chromosomal ampC beta-lactamase gene of Pseudomonas aeruginosa and the Enterobacteriaceae. We tested this probe against a variety of Enterobacteriaceae, and a series of 23 P. aeruginosa by dot-blots and selected Southern blots. This probe is an alternative or supplement to enzyme studies for characterizing the class of a Gram-negative rod's beta-lactamase and is a useful tool for studies of Pseudomonas beta-lactamase regulation.