• Citer

The relationship of ribonucleic acid to the in vitro incorporation of radioactive glycine into the proteins of reticulocytes

par Koritz, Seymour S.B.;Chantrenne, Hubert
Référence Biochimica et biophysica acta, 13, C, page (209-215)
Publication Publié, 1954

Article révisé par les pairs

• Accès en ligne
• Détails
• Contenu
• Statistiques

Résumé :

1. 1. The rate of incorporation of 14C-glycine into the proteins of reticulocytes and their RNA content have been determined as a function of the development and disappearance of a phenyl-hydrazine induced reticulocytosis in rabbits. 2. 2. The peak of labelled amino acid incorporation occurred 1 to 2 days before the RNA peak. 3. 3. The formation of carbonic anhydrase, hemoglobin and peptidase corresponds quite closely with the RNA curve rather than with the 14C glycine incorporation curve. 4. 4. These results are interpreted to indicate that RNA is not involved in the initial incorporation of amino acids into proteins but rather mediates the differentiation of a "primary" protein into its final stereospecific, biologically active form. © 1954.