par Fontigny, Anne;Billen, Gilles ;Vives-Rego, Jose
Référence Estuarine, coastal and shelf science, 25, 1, page (127-133)
Publication Publié, 1987
Article révisé par les pairs
Résumé : The response of the exoproteolytic activity of seawater to proteolytic inhibitors suggests that metalloproteases are the main enzymes involved. The Km of exoproteolytic enzymes for the hydrolysis of indigenous proteins in coastal north seawater has been evaluated as 80 μg l-1 and the maximum rate of proteolysis lies in the range 0·1-0·35 μgC l-1-enzymatic-unit-1. Enrichment experiments suggest that both species selection and metabolic regulation may play a role in the exoproteolytic activity/biomass ratio. However, in situ exoproteolytic activity/biomass ratios observed in a broad range of natural marine environments lie in a very narrow range, which is intermediate between those observed after amino acid or protein enrichment. © 1987.