par Kettmann, Richard 
;Portetelle, Daniel ;Mammerickx, Marc;Cleuter, Yvette ;Dekegel, Daniel ;Galoux, Michel;Ghysdael, Jacques ;Burny, Arsène ;Chantrenne, Hubert
Référence Proceedings of the National Academy of Sciences of the United States of America, 73, 4, page (1014-1018)
Publication Publié, 1976
;Portetelle, Daniel ;Mammerickx, Marc;Cleuter, Yvette ;Dekegel, Daniel ;Galoux, Michel;Ghysdael, Jacques ;Burny, Arsène ;Chantrenne, Hubert Référence Proceedings of the National Academy of Sciences of the United States of America, 73, 4, page (1014-1018)
Publication Publié, 1976
Article révisé par les pairs
| Résumé : | Short term cultures of bovine leukemic lymphocytes release virus particles with biochemical properties of RNA oncogenic viruses. These particles, tentatively called bovine leukemia virus (BLV), have a high molecular weight RNA reverse transcriptase complex and a density of 1.155 g/ml in sucrose solutions. Molecular hybridizations between BLV [3H] cDNA and several viral RNAs show that BLV is not related to Mason Pfizer monkey virus, simian sarcoma associated virus, feline leukemia virus, or avian myeloblastosis virus. These results were confirmed by hybridization between BLV 70S RNA and [3H] cDNA synthesized in the various viruses tested. The high preference of BLV reverse transcriptase for Mg++ as the divalent cation suggests that BLV might be an atypical mammalian leukemogenic 'type C' virus. DNA DNA hybridization studies using BLV [3H] cDNA as a probe strongly suggest that the DNA of bovine leukemic cells contains viral sequences that cannot be detected in normal bovine DNA. |
