Résumé : We investigated roosting and hunting site fidelity of Daubenton's bats Myotis daubentonii (Kuhl, 1817) in the Forêt de Soignes, an old-stand forest dominated by 150-200-year-old beeches, during the summers of 2003 and 2004. Roosting behaviour and hunting activity over ponds of adult females and juveniles were monitored using radio-telemetry. Eighteen roosts were located, all in natural cavities. The bats occupied a limited number of trees located in a specific and small roosting area. This roost aggregation was not linked to the distribution of hollow trees. Furthermore, whereas all 11 ponds in the study area were exploited by Daubenton's bats, monitored individuals were limited to two ponds to which they showed high fidelity. These two ponds were not the closest to the roosting area. Overall, these results show that at least for the time we conducted our experiment, female and juvenile Daubenton's bats are highly faithful to specific roosting and hunting grounds. © 2007 Deutsche Gesellschaft für Säugetierkunde.