par Couturier, Martine 
;Janssens, J.;Bex, Françoise ;Desmyter, Aline ;Bonnevalle, I.
Référence Molecular & general genetics : MGG, 169, 1, page (113-116)
Publication Publié, 1979-01
;Janssens, J.;Bex, Françoise ;Desmyter, Aline ;Bonnevalle, I.Référence Molecular & general genetics : MGG, 169, 1, page (113-116)
Publication Publié, 1979-01
Article révisé par les pairs
| Résumé : | In this paper, we report the construction in vitro of chimerae between lambdoid replacement vectors (Murray et al., 1977) and the miniF Apr plasmid: pSC138 (Timmis et al., 1975). λF recombinants were shown to be chimerae between the λ and the F replicons. By genetical tests, we have demonstrated that both λ and F replication mechanisms are functional: the λF recombinant behaves as a non defective plaque forming phage on λ sensitive bacteria and establishes itself as a stable plasmid on recA F- homoimmune bacteria. In the extra-chromosomal state, the λF recombinant apparently retains the controlled autonomous replication and the FI incompatibility characteristics of the F plasmid. The potential experimental uses of these phages are discussed. © 1979 Springer-Verlag. |
