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Relationship between the N function of bacteriophage λ and host RNA polymerase

par Ghysen, Alain ;Pironio-Cojazzi, Marina
Référence Journal of Molecular Biology, 65, 2, page (259-272)
Publication Publié, 1972-03

Article révisé par les pairs

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Résumé :

A bacterial mutation (ron) has been isolated which prevents growth of some λ lines. These lines were found to carry a mutation in the N gene, mar, which remains undetected in ron+ strains. A mar phage in a ron strain behaves as an N- mutant both as concerns complementation properties and viral messenger RNA synthesis. The ron mutation maps between two mutations which affect the RNA polymerase: rif-r and stl-r. Complementation experiments confirm that all three mutations (ron, rif-r and stl-r) lie in the same cistron, namely, the structural gene for the β subunit of RNA polymerase. The ron-mar relationship has been analysed; results suggest a direct interaction between the viral N product and the bacterial RNA polymerase. © 1972.