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In vitro modulation of human gingival epithelial cell attachment and migration by minocycline-HCI

par Vanheusden, Alain;Rompen, Eric E.H.;Nusgens, Betty;Lapiere, Charles ;Goffinet, Gérhard;Zahedi, Sharam
Référence Journal of periodontal research, 33, 3, page (377-385)
Publication Publié, 1998-04

Article révisé par les pairs

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Résumé :

Although the influence of tetracyclines on periodontal connective tissue cells has been the topic of many in vitro and in vivo studies, data regarding their effects on gingival epithelial cells are scarce. The present in vitro study was designed to examine the influence of minocycline, a semi-synthetic analog of tetracycline, on human gingival keratinocyte (HGK) attachment and migration. Attachment tests were performed with HGK prelabeled by tritiated amino-acids. Increasing concentrations of minocycline (10, 50, 100 μg/ml) in the medium produced no significant modification of cell adhesion kinetics compared to control conditions, except for 100 μg/ml which statistically significantly (p<0.05) reduced the number of attached cells beyond 6 h. A 24-h cell preincubation in 10 μg/ml of minocycline did not alter the kinetics of HGK attachment. Scanning electron microscopic observations of attached HGK showed that the presence of 10 μg/ml of minocyline in the "attachment medium" induced the production of multiple filopodial extensions. Migration tests in Boyden chambers for 40 h demonstrated that HGK preincubation for 24h in a 10 μg/ml minocycline-HC1 solution increased significantly (p<0.005) cell migation towards a gradient of fetal calf serum. The presence of 10 μg/ml of minocycline in contact with the keratinocytes in the upper compartment of the migration chambers also produced a significant (p<0.005) result. In contrast. the presence of minocycline in the lower compartments did not produce any chemoattractive effect. Within the limits of their significance, these results suggest that, at concentrations not beyond 50 μg/ml, minocycline could fasten the periodontal wound coverage by epithelial cells and allow the normal reformation of a junctional epithelium. © Munksgaard, 1998.