• Citer

Anti-alprenolol anti-idiotypic antibodies bind to β-adrenergic receptors and modulate catecholamine-sensitive adenylate cyclase

par Schreiber, Alain A.B.;Couraud, Pierre Olivier;Andre, Claudine;Vray, Bernard ;Strosberg, Arthur
Référence Proceedings of the National Academy of Sciences of the United States of America, 77, 12 II, page (7385-7389)
Publication Publié, 1980

Article révisé par les pairs

• Accès en ligne
• Détails
• Contenu
• Statistiques

Résumé :

Rabbit antibodies induced against alprenolol, a potent β-adrenergic antagonist, bind to other antagonists and, with less avidity, to catecholamine agonists. Anti-idiotypic antibodies were raised against the anti-alprenolol immunoglobulins. The anti-idiotypic antibodies specifically bind and agglutinate turkey erythrocytes; this is not observed for human or sheep erythrocytes, which are devoid of β-adrenergic receptors. The anti-idiotypic antibodies compete with (-)-[ 3H]-dihydroalprenolol for binding on the β-adrenergic receptors on purified turkey erythrocyte membranes. The binding to the membrane-bound receptors is prevented by preincubation of the anti-idiotypic antibodies with their immunogen, the anti-alprenolol immunoglobulins. The binding to the receptor is not merely passive: the anti-idiotypic antibodies stimulate basal adenylate cyclase activity [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] and enhance adenylate cyclase activation by catecholamine. These observations support the notion that anti-idiotypic antibodies may constitute an 'internal image' of the original antigen and may mimic its biological effects.