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Study of the uptake of ribonuclease by onion root-tip cells

par Bhide, S.V.;Brachet, Jean
Référence Experimental cell research, 21, 2, page (303-315)
Publication Publié, 1960-11

Article révisé par les pairs

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Résumé :

The following observations have been made concerning the uptake of ribonuclease by living onion roots: 1. 1. The penetration of RNAse in roots separated from the bulb is 37 per cent less than that observed in the case of roots still attached to the bulb. 2. 2. The uptake of RNAse at 4 °C is 40 per cent less than at 20 °C. 3. 3. Dinitrophenol (DNP) has no effect on the uptake, which is, however, inhibited by cyanide. 4. 4. Buffered DNP, as well as phosphate buffer alone, has an inhibitory effect on the penetration. 5. 5. There is no competition for penetration between ribonuclease and cytochrome c. 6. 6. The ions K +, Na +, Ca 2+ and Mg 2+ exert an inhibitory effect on the penetration of RNAse. 7. 7. Sixty per cent of the RNAse which has penetrated in onion roots can be found in cell debris, 25 per cent in the mitochondria and 15 per cent in the microsomcs. 8. 8. Only 50 per cent of the RNAse which has penetrated is released by a 24-hour washing. The mechanism of the penetration of RNAsc in onion roots is discussed. © 1960.