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Anomalous sedimentation behaviour of the e-coli ribosomal system: 50S-30S ⇄ 50S + 30S

par Shcherbukhin, Victor V.V.;Guermant, Claude
Référence Biophysical chemistry, 3, 1, page (21-34)
Publication Publié, 1975

Article révisé par les pairs

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Résumé :

An experimental and theoretical study has been made into the effect of association-dissociation reactions on the sedimentation of the E. coli ribosomal system 50S-30S ⇄ 50S + 30S. It has been found that. (a) the sedimentation pattern is strongly dependent on the rotor speed;. (b) the ratio of components as measured using high-speed ultracentrifugation (30000-40000 r.p.m.) is independent of rotor speed; and. (c) the speed of ultracentrifugation has a strong effect on the sedimentation coefficient of the ribosomal system as determined by the mean square second moment. The results of this paper demonstrate that ribosome sedimentation at low-speed ultracentrifugation is affected by some artefactual processes. A theoretical analysis of the experimental findings has shown that the observed effects cannot be attributed to the effect of the association-dissociation reaction not to the pressure dependence of the equilibrium constant of that reaction. On the other hand, at high-speed ultracentrifugation the ribosomal system sediments as a heterogeneous mixture of non-interacting components. Consequently, the shape of the boundary in this case will reflect the equilibrium composition of the ribosomal system. © 1975.