par Brachet, Jean 
;Cape, Marianne ;Decroly, Marc ;Hanocq Quertier, Jacqueline ;Six, Nicole
Référence Developmental biology, 3, 4, page (424-443)
Publication Publié, 1961-08
;Cape, Marianne ;Decroly, Marc ;Hanocq Quertier, Jacqueline ;Six, Nicole Référence Developmental biology, 3, 4, page (424-443)
Publication Publié, 1961-08
Article révisé par les pairs
| Résumé : | The biochemistry of amphibian eggs treated with mercaptoethanol ( M 100) under conditions which completely inhibit morphogenesis has been studied. It has been found that: 1. 1. Mercaptoethanol undergoes a fairly rapid oxidation under the experimental conditions. 2. 2. The acid-soluble -SH groups content increases in mercaptoethanol-treated embryos. 3. 3. Treatment with mercaptoethanol leads to a moderate decrease in the oxygen consumption and has no marked effect on the ATP content. 4. 4. The block in development induced by mercaptoethanol cannot be explained on the basis of a stimulation of catheptic activity. 5. 5. Mercaptoethanol does not immediately inhibit the incorporation of thymidine into DNA, of uridine into nuclear nucleic acids, and of leucine into the proteins of the treated embryos. Inhibition of nucleic acid and protein synthesis can be observed when the effects of mercaptoethanol on morphogenesis become irreversible. The significance of these results is discussed. © 1961. |
