par Achour, Ammar;Mbika, Jean Pierre;Willer, Andreas;Snart, Ronald;Bizzini, Bernard;Carelli, Claude;Zagury, Daniel;Picard, Odile;Burny, Arsène 
Référence Vaccine, 11, 7, page (699-701)
Publication Publié, 1993
Référence Vaccine, 11, 7, page (699-701)
Publication Publié, 1993
Article révisé par les pairs
| Résumé : | Cytotoxic T cells are the main antigen-specific effector cells of the cellular immune system and MHC class I restricted cytotoxic T-lymphocyte (CTL) responses in mice, acting against the HIV-1 envelope protein, are known to be predominantly directed against an amino acid sequence in the third hypervariable domain. We have investigated the epitope specificity of anti-HIV-1 CTL in healthy human volunteers inoculated with a recombinant vaccinia expressing the HIV-1 gp160 envelope gene. Their isolated lymphocytes were stimulated in vitro with autologous HIV-1 infected cells. Our results show that immunization with recombinant virus is able to generate virus-specific CTLs to the HIV-1 gp160 envelope protein and to a 15-residue synthetic peptide corresponding to a highly variable region of the envelope p18(IIIB). The CTL response was restricted by class I MHC molecules HLA-A2 and A3 that commonly occur in the human population. © 1993. |
