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Étude au microscope électronique de l'action de l'hydroxyurée sur les embryons. II. Embryons d'amphibiens

par Geuskens, Maurice
Référence Experimental cell research, 52, 2-3, page (621-631)
Publication Publié, 1968-10

Article révisé par les pairs

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Résumé :

Pleurodeles waltlii eggs were treated with hydroxyurea (1 mg/ml or 2 mg/ml) for 22 h, from the 2 cells stage onwards. Controls (aged blastulae) and hydroxyurea treated embryos (blocked at the morula-young blastula stage) were studied with the electron microscope. The nuclei of normal blastulae contain many nucleolar corpuscles (about 1 μ in diameter) of fibrillar structure, or larger nucleoli of partially granular structure. In hydroxyurea treated embryos, the nucleolar components have segregated and the chromatin is condensed in a coarse network. Granules similar to cytoplasmic glycogen particles can be seen in the nuclear sap; this abnormal localization is the result of mitotic abnormalities. The alterations observed in hydroxyurea treated eggs are consistent with the hypothesis of a direct action of this substance on DNA itself. © 1968.