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DNA polymerase γ, cytochrome c oxidase and mitochondrial integrity in rabbit spleen lymphocytes stimulated with concanavalin A

par Hardt, Nadine;Villani, Giuseppe ;De, Arnab K;Vanheule, L.;Spadari, Silvio
Référence Experimental cell research, 127, 2, page (269-276)
Publication Publié, 1980

Article révisé par les pairs

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Résumé :

The activities of two mitochondrial enzymes: DNA polymerase γ and cytochrome c oxidase, have been compared with the kinetics of nuclear DNA synthesis and levels of the nuclear DNA polymerases α and β in rabbit spleen lymphocytes untreated or stimulated with concanavalin A (ConA). At early initiation of the culture, before the maximum increase of both the replicative α-polymerase and nuclear DNA synthesis, a simultaneous enhancement in the activities of the two mitochondrial enzymes was observed, just preceding and paralleling the expansion of the ATP pool. It is suggested that this is a reflection of increased mitochondrial activity providing the energy which results in an elevation of the concentration of dNTPs to values which are optimal for the replicative α-polymerase. During the later stages of incubation of lymphocytes, the activities of both DNA polymerase γ and cytochrome c oxidase decrease, paralleling a progressive loss of integrity of the mitochondrial structures within an increasing proportion of the lymphocyte population. © 1980.