par De Sutter, Danielle ;Van De Vyver, Gisèle
Référence Developmental and comparative immunology, 3, C, page (389-397)
Publication Publié, 1979
Article révisé par les pairs
Résumé : Two cell fractions can be isolated from strains of the fresh-water sponges Ephydatia fluviatilis by means of Ficoll gradients. The densest fraction consists of pure archaeocytes; the least dense is very rich in choanocytes and free from archaeocytes. Both fractions aggregate; but archaeocyte aggregates undergo a complete process of reconstitution whereas choanocyte aggregates fail to settle. Both were tested for their allogeneic recognition properties by mixing 3 different proportions of homologous cell fractions isolated from incompatible strains. In equicellular ratios, dominance of one strain or survival of both depending on the paired strain, whereas in unequal ratios, dominance of the most concentrated strain appears to be the rule. Phagocytosis in the aggregates, is enhanced in allogeneic combinations compared to unmixed control samples. © 1979 Pergamon Press Ltd. All rights reserved.