par Barlow, Patricia 
;Van der Zwalmen, P.;Schoysman, Robert ;Leroy, Fernand
Référence Molecular reproduction and development, 30, 4, page (385-389)
Publication Publié, 1991-12
;Van der Zwalmen, P.;Schoysman, Robert ;Leroy, Fernand Référence Molecular reproduction and development, 30, 4, page (385-389)
Publication Publié, 1991-12
Article révisé par les pairs
| Résumé : | The usefulness of opening the zona pellucida by partial zona dissection (PZD) to enhance fertilization of mature mouse oocytes was studied after insemination with three types of semen: normal and diluted semen and semen from long-term-vasectomized males. Zona opening did not by itself induce parthenogenetic cleavage of mature oocytes and did not significantly increase mono- and polyspermic fertilization of oocytes inseminated with normal semen. While a fertilization rate of 62% was obtained among intact oocytes, of which 4.5% were polyspermic, a 66.8% fertilization rate was observed among PZD oocytes, 6.3% of which were polyspermic. However, after using diluted semen, only 54 of 193 intact oocytes were fertilized (28%), and PZD improved the fertilization rate to 65.4%. Cleavage rate of nonmanipulated oocytes inseminated with abnormal semen from vasectomized males was dramatically decreased in comparison with those inseminated with normal semen (7.6% vs. 65%). PZD induced a moderate but significant improvement of fertilization performance when using this abnormal semen (19.6%). |
