par Vray, Bernard 
;Hoebeke, Johan;Saint-Guillain, Marc ;Leloup, Robert;Strosberg, Arthur
Référence Scandinavian journal of immunology, 11, 2, page (147-153)
Publication Publié, 1980-02
;Hoebeke, Johan;Saint-Guillain, Marc ;Leloup, Robert;Strosberg, Arthur Référence Scandinavian journal of immunology, 11, 2, page (147-153)
Publication Publié, 1980-02
Article révisé par les pairs
| Résumé : | The authors describe a new quantitative fluorimetric assay for phagocytosis of bacteria. A suspension of fluorescein-labelled bacteria (Micrococcus lysodeikticus) is mixed and incubated with phagocytes. After fixation with paraformaldehyde, the excess non-phagocytosed and adsorbed bacteria are lysed with a solution of lysozyme in phosphate-buffered saline. After washing of the phagocytes, the fluorescence of those that have ingested the labelled bacteria is measured with a spectrofluorimeter. The authors report results obtained with different types of phagocytes which show that this method allows sensitivity, saturation and kinetic studies and the calculation of a phagocytic index. |
