par Perry, Robert R.P.;Hell, A.;Errera, Maurice Leo
Référence Biochimica et biophysica acta, 49, 1, page (47-57)
Publication Publié, 1961-04
Référence Biochimica et biophysica acta, 49, 1, page (47-57)
Publication Publié, 1961-04
Article révisé par les pairs
Résumé : | Nucleoli of HeLa cells growing in tissue culture were selectively irradiated with an u.v. microbeam and the subsequent incorporation of [3H]cytidine into the ribonucleic acid of the nucleolus, extra-nucleolar parts of the nucleus, and the cytoplasm, was studied by autoradiography. Comparison with cells containing normal nucleoli revealed that approximately 2 3 of the cytoplasmic incorporation is directly dependent on the nucleolus, but that less than 1 3 of the incorporation into extra-nucleolar parts of the nucleus is dependent on the nucleolus. These results when combined with quantitative kinetic studies of cytidine incorporation strongly support the hypothesis that a major part of the ribonucleic acid of the cytoplasm is synthesized in the nucleolus. © 1961. |