par Marchetti, Piero;Castedo, Maria;Susin, Santos;Zamzami, Naoufal;Hirsch, Tamara;Macho, Antonio;Haeffner, Astrid;Hirsch, François;Geuskens, Maurice ;Kroemer, Guido
Référence The Journal of experimental medicine, 184, 3, page (1155-1160)
Publication Publié, 1996-09
Article révisé par les pairs
Résumé : In a number of experimental systems, the early stage o the apoptotic process, i.e., the stage that precedes nuclear disintegration, is characterized by the breakdown of the inner mitochondrial transmembrane potential (ΔΨ(m)). This ΔΨ(m) disruption is mediated by the opening of permeability transition (PT) pores and appears to be critical for the apoptotic cascade, since it is directly regulated by Bcl-2 and since mitochondria induced to undergo PT in vitro become capable of inducing nuclear chromatinolysis in a cell-free system of apoptosis. Here, we addressed the question of which apoptotic events are secondary to mitochondrial PT. We tested the effect of a specific inhibitor of PT, bongkrekic acid (BA), a ligand of the mitochondrial academic nucleotide translocator, on a prototypic model of apoptosis; glucocorticoid-induced thymocyte death. In addition to abolishing the apoptotic ΔΨ(m) disruption, BA prevents a number of phenomena linked to apoptosis: depletion of nonoxidized glutathione, genetic generation of reactive oxygen species, translocation of NFκB, exposure of phosphatidylserine residues on the outer plasma membrane, cytoplasmic vacuolization, chromatin condensation, and oligonucleosomal DNA fragmentation. BA is also an efficient inhibitor of p53- dependent thymocyte apoptosis induced by DNA damaged. These data suggest that a number of apoptotic phenomona are secondary to PT. In addition, we present data indicating that apoptotic ΔΨ(m) disruption is secondary to transcriptional events. These data connect the PT control point to the p53- and ICE/Ced 3-regulated control points of apoptosis and place PT upstream of nuclear and plasma membrane features of PCD.

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