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The behaviour of cow blastocyst in vitro: Cinematographic and morphometric analysis

par Massip, Alban;Mulnard, Jacques ;Vanderzwalmen, Pierre;Hanzen, C.;Ectors, F.J.
Référence Journal of anatomy, 134, 2, page (399-405)
Publication Publié, 1982

Article révisé par les pairs

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Résumé :

The behaviour of the cow blastocyst in vitro was studied by time-lapse cinematography and analysed by morphometry. Three types of behaviour were observed: continuous expansion followed by hatching; discontinuous expansion interrupted by few contractions and followed by hatching; discontinuous expansion interrupted by several rapid contractions without hatching. This demonstrated that the pulsatile activity of the blastocyst is not a necessary condition of hatching but also that only a moderate pulsatile activity is compatible with normal hatching. The time of hatching in vitro corresponded approximately with the time of zona loss in vivo (9-10 days). Rupture of the zona occurred at any point of the trophoblast layer. Hatching by herniation through a reduced opening of the zona was occasionally observed. The behaviour of the embryos from a particular animal was very similar but differences were noted between embryos from different animals.