par Liénard, P.;De Mees, Christelle 
;Dreze, Pierre Luc ;Dieu, Marc;Dierick, Jean-Francois ;Raes, Martine;Szpirer, Josiane ;Szpirer, Claude
Référence Biochimie, 88, 10, page (1409-1417)
Publication Publié, 2006
;Dreze, Pierre Luc ;Dieu, Marc;Dierick, Jean-Francois ;Raes, Martine;Szpirer, Josiane ;Szpirer, Claude Référence Biochimie, 88, 10, page (1409-1417)
Publication Publié, 2006
Article révisé par les pairs
| Résumé : | This work shows that the proximal promoter of the mouse Afp gene contains a Ku binding site and that Ku binding is associated with down-regulation of the transcriptional activity of the Afp promoter. The Ku binding site is located in a segment able to adopt a peculiar structured form, probably a hairpin structure. Interestingly, the structured form eliminates the binding sites of the positive transcription factor HNF1. Furthermore, a DNAse hypersensitive site is detected in footprinting experiments done with extracts of AFP non-expressing hepatoma cells. These observations suggest that the structured form is stabilised by Ku and is associated with extinction of the gene in AFP non-expressing hepatic cells. |
