par Negrutiu, Ioan ;Beeftink, F.;Jacobs, Michel
Référence Plant Science Letters, 5, 5, page (293-304)
Publication Publié, 1975-11
Article révisé par les pairs
Résumé : Induction and growth of callus from seeds, stems and pieces of leaf of Arabidopsis thaliana were successful on a Gamborg B5 medium and on a modified B5 medium (PG2). Actively growing calluses have been obtained on agar and in liquid cultures. Cell suspension cultures were establiched on the same culture media, with cell densities up to 1.6·106 cells/ml. A culture system is described which enables us to use large cell aggregates as a continuous source of single cells and small cell aggregates (containing up to 10 cells) for mutagenesis, plating and protoplast production. Arabidopsis plants were regenerated from young calluses grown in solid or liquid cultures, by a sequential transfer from a medium (PG3), where shoot initiation takes place, to a PG4 medium which induces root formation. The morphogenetic potential appeared to depend on the age of the callus in culture. A wide range of heteroploidy has been observed in tissue cultures. The influence of factors such as the age of the callus, the type of organ used to induce calluses, the hormone ratio and the composition of the medium was determined. A procedure for the isolation of protoplasts from leaves and cell cultures is described. © 1975.