par Grosjean, Henri ;Werenne, John ;Chantrenne, Hubert
Référence Biochimica et biophysica acta, N. Nucleic acids and protein synthesis, 166, 3, page (616-627)
Publication Publié, 1968-10
Article révisé par les pairs
Résumé : Proflavine (3,6-diaminoacridine) binds strongly to transfer RNA (tRNA) from Escherichia coli. Direct evidence is obtained by ultracentrifugation, gel electrophoresis and molecular filtration of the complex. Studies on the stability of the tRNA-proflavine complex as a function of temperature show that strong binding depends on the secondary structure of the nucleic acid. Proflavine stabilizes the macromolecule against thermal denaturation. The results are comparable to those obtained with proflavine and DNA. The complexes of proflavine with tRNA and with DNA are probably of a similar nature. © 1968.