• Citer

Single-molecule force spectroscopy and imaging of the vancomycin/D-Ala-D-Ala interaction.

par Gilbert, Yann;Deghorain, Marie ;Wang, Ling;Xu, Binghe;Pollheimer, Philipp D;Gruber, Hermann J;Errington, Jeff;Hallet, Bernard;Haulot, Xavier;Verbelen, Claire;Hols, Pascal;Dufrêne, Yves F
Référence Nano letters, 7, 3, page (796-801)
Publication Publié, 2007-03

Article révisé par les pairs

• Détails
• Contenu

Résumé :

The clinically important vancomycin antibiotic inhibits the growth of pathogens such as Staphylococcus aureus by blocking cell wall synthesis through specific recognition of nascent peptidoglycan terminating in D-Ala-D-Ala. Here, we demonstrate the ability of single-molecule atomic force microscopy with antibiotic-modified tips to measure the specific binding forces of vancomycin and to map individual ligands on living bacteria. The single-molecule approach presented here provides new opportunities for understanding the binding mechanisms of antibiotics and for exploring the architecture of bacterial cell walls.