par Mauffret, Aourell;Rottiers, André;Federle, Thomas;Gillan, David
;Hampel, Miriam;Blasco, Julian;Temara, Ali 
Référence Environment international, 35, 6, page (885-892)
Publication Publié, 2009-08


Référence Environment international, 35, 6, page (885-892)
Publication Publié, 2009-08
Article révisé par les pairs
Résumé : | An innovative biodegradation test system was developed in order to fill the current gap for cost effective and environmentally relevant tools to assess marine biodegradability. Glass beads were colonized by a biofilm in an open flow-through system of seawater with continuous pre-exposure to Linear Alkylbenzene Sulfonate (LAS) (20 μg/L). Thereafter, such colonized beads were added as inoculum in different test systems. [14C]-LAS (5-100 μg/L) was added and primary and ultimate biodegradation were assessed. The bacterial density collected on the beads (109 bact./mL beads) was ca. 3 orders of magnitude higher than the typical seawater content. The LAS mineralization lag phase duration decreased from 55 to < 1 days and the mineralization extent increased from 53 to 90% as the colonized beads volume increased from 10 to 275 mL. This is the first demonstration of marine bacteria's ability to mineralize LAS. On the opposite, less than 13% LAS was mineralized in seawater only. The colonized beads possibly enhanced the probability to encounter the full degraders' consortium in a low volume of seawater (100 mL). © 2009 Elsevier Ltd. All rights reserved. |