Résumé : The separation of enantiomers of several 'bis- and tris-Tröger's bases' by HPLC on commercially available chiral stationary phase Whelk O1 is described for the first time. The observed structure-enantioselectivity relationships are in agreement with the previously established molecular recognition model. For all 'bis- and tris-Tröger's bases' studied, satisfactory to excellent enantioselectivities were observed. © 2009 Elsevier Ltd. All rights reserved.