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Novel GTP-binding proteins in plasma membranes and zymogen granule membranes from rat pancreas and in pancreatic AR 4-2J cell membranes

par Lambert, Monique;Bui, Ngoc Diem ;Christophe, Jean
Référence FEBS letters, 271, 1-2, page (19-22)
Publication Publié, 1990

Article révisé par les pairs

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Résumé :

Photoaffinity labeling with [α-32P]GTP allowed to detect a 54 kDa GTP-binding protein in rat pancreatic plasma membranes and in pancreatic AR 4-2J cell membranes. Like the 42 and 48 kDa G(s)α subunits and the 41 kDa G(i)α subunit, this protein was absent from zymogen granule membranes. Contrastingly, a new 28 kDa GTP-binding protein (detected by [α-32P]GTP binding on immobilized proteins) and a 25 kDa protein (ADP-ribosylated by botulinum toxin D) were found in all three membrane preparations. This is to our knowledge the first report on GTP-binding proteins in zymogen granule membranes.