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Effects of X rays on nucleic acid metabolism during cleavage and gastrulation in Pleurodeles waltlii eggs

par Alexandre, Henri
Référence Journal of Embryology and Experimental Morphology, 32, 1, page (147-157)
Publication Publié, 1974

Article révisé par les pairs

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Résumé :

X irradiation produces a rapid and substantial decrease in DNA synthesis in cleaving eggs of Pleurodeles, as shown by thymidine incorporation experiments; this decrease is presumably due to the arresting of the cell cycle in many blastomeres. DNA replication is partly resumed at the late blastula stage after irradiation at the 2 to 4 cell stage. The present work demonstrates a high radiosensitivity of the metabolic pathway leading to the incorporation of uridine into DNA; this could be the result of the inhibition of the activity or the synthesis of such enzymes as ribonucleotide reductase, thymidylate synthetase and CTP synthetase. The radiosensitivity of this metabolic pathway becomes lower after gastrulation. Finally, irradiation strongly inhibits the synthesis of new RNA species at gastrulation, but seems to have no effect on heterodisperse RNA (mRNA) synthesis during cleavage.