Article révisé par les pairs
Résumé : Biphenyl dioxygenase from the psychrotolerant bacterium Pseudomonas sp. strain Cam-1 (BPDOCam-1) was purified and found to have an apparent kcat for biphenyl of 1.1 ± 0.1 s-1 (mean ± standard deviation) at 4°C. In contrast, BPDOLB400 from the mesophile Burkholderia xenovorans LB400 had no detectable activity at this temperature. At 57°C, the half-life of the BPDOCam-1 oxygenase was less than half that of the BPDOLB400 oxygenase. Nevertheless, BPDOCam-1 appears to be a typical Pseudomonas pseudoalcaligenes KF707-type dioxygenase. Copyright © 2008, American Society for Microbiology. All Rights Reserved.