Résumé : Fasciated shoots of Pratia nummularia (Campanulaceae) were obtained from the leafy galls of the plant by infection with a Gram-positive actinomycete Rhodococcus fascians. The major secondary metabolites in R. fascians infected shoot cultures of P. nummularia were identified as polyacetylenes (lobetyol, lobetyolin and lobetyolinin) and flavonoids (diosmin and linarin). The concentration of lobetyolin in R. fascians infected shoots (0.2%, dry weight) was almost a half of the concerted in non-infected shoots (0.49%, dry weight). The other polyacetylene and flavonoid concentrations were similar in infected and non-infected shoots. There was little difference in the pattern of the major secondary metabolites in R. fascians infected (fasciated) and non-infected P. nummularia shoot cultures.