par Delsaute, Maud;Berlemont, Renaud;Dehareng, Dominique;Van Elder, Dany ;Galleni, Moreno;Bauvois, Cédric
Référence Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 69, 8, page (828-833)
Publication Publié, 2013-08
Article révisé par les pairs
Résumé : RBcel1 is an endoglucanase belonging to glycoside hydrolase family 5 subfamily 5 (GH55) that was recently identified from a soil metagenome library from the Antarctic. Unlike its closest structural homologue (Cel5A from Thermoascus aurantiacus), this enzyme was reported to be able to catalyze transglycosylation reactions and has putatively been implicated in the bacterial cellulose-synthesis process. Here, the structure of RBcel1 at 1.4Å resolution, solved by molecular replacement, is reported. The structure and putative substrate-binding site are described and compared with those of other GH55 subfamily members. © 2013 International Union of Crystallography.