par Ommen, Gert-jan;Arnberg, Annika A.C.;Baas, Frank;Brocas, Huguette;Sterk, Andrè;Tegelaers, Wil W.H.H.;Vassart, Gilbert ;Vijlder, Jan J.J.M.
Référence Nucleic acids research, 11, 8, page (2273-2285)
Publication Publié, 1983-04
Référence Nucleic acids research, 11, 8, page (2273-2285)
Publication Publié, 1983-04
Article révisé par les pairs
Résumé : | We have cloned overlapping segments of the human thyroglobulin gene from a genomic cosmid library. Restriction mapping and electron microscopy show that a region of 38 kb at or near the 3′-end of this gene encodes only 850 nucleotides or 10% of the messenger RNA (mRNA) sequence. The region contains five exons of 130-210 nucleotides, split by introns of 1 to 15-17 kb. This represents the lowest ratio of coding to non-coding DNA (2.2%) found thusfar in any eukaryotic gene. Blot hybridization under non-stringent conditions shows the presence of only one copy of this gene in the human genome and the absence of other closely related sequences. © 1983 IRL Press Limited. |