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L'oxydation bismuthique des corticostéroïdes urinaires: application à la mesure de la sécrétion de cortisol par dilution isotopique

par Copinschi, Georges ;Cornil, Arnaud ;Franckson, Jean
Référence Clinica chimica acta, 11, 4, page (346-353)
Publication Publié, 1965-04

Article révisé par les pairs

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Résumé :

A new method is described for the measurement of cortisol secretion rate by the isotope dilution technique. The main steps of the procedure are a bismuthate oxidation of the urinary metabolites of cortisol, an alumina column chromatography and the determination of the specific activity of the pooled major metabolites of cortisol. This procedure avoids the difficulties encountered in the methods using paper chromatography. For ten normal adult males, cortisol secretion rate was 22.0 mg/24 h with a standard deviation of 3.1 mg. These results are in fair agreement with the data recorded in the literature. © 1965.