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Fatty acid synthesis by the liver perfused with deuterated and tritiated water

par Wadke, Manohar;Brunengraber, Henri ;Lowenstein, John J.M.;Dolhun, John;Arsenault, Gilles
Référence Biochemistry, 12, 14, page (2619-2624)
Publication Publié, 1973

Article révisé par les pairs

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Résumé :

Fatty acid synthesis has been studied in livers of rats perfused with 10% D2O and with >90% D2O. The number of deuterium atoms incorporated in 100% D2O is 22.3 and 24.9 per molecule of newly synthesized palmitate and stearate respectively. The result for palmitate agrees with the result obtained by Jungas with rat adipose tissue under quite different conditions ((1968), Biochemistry 10, 3717). Mass spectrometric measurements of deuterium content were used to measure the rate of fatty acid synthesis. The results so obtained agree well with results obtained by measuring tritium incorporation from 3H2O. Mass spectrometric examination of fatty acids synthesized in the presence of high concentrations of D2O provides direct information concerning the extents to which a fatty acid is formed by de novo synthesis and by chain elongation of other fatty acids. Stearate is synthesized at about 40% the rate of palmitate. Of the stearate formed about 97% is made by de novo synthesis. (An alternative interpretation of the last result is that the chain elongation mechanism uses only palmitate synthesized de novo and little or no preexisting palmitate.).