par Herion, Pascal 
;De Coen, Jean-Louis
Référence Molecular immunology, 23, 2, page (209-215)
Publication Publié, 1986
;De Coen, Jean-Louis Référence Molecular immunology, 23, 2, page (209-215)
Publication Publié, 1986
Article révisé par les pairs
| Résumé : | A rat × mouse hybridoma line producing a monoclonal antibody against leu5 enkephalin has been obtained. The monoclonal antibody belongs to the IgG2b class and has an affinity constant of 8.0 × 108 M-1 at 4°C. This antibody exhibits ~40% cross-reactivity with 1-6 dynorphin but very weak cross-reactivities with met5 enkephalin (1.4%), 1-13 dynorphin (1.3%) and β-endorphin (0.0045%). The detailed study, by competitive assay, of the interaction between this antibody and various enkephalin derivatives shows that the carboxy-terminal part of the molecule and, particularly, the leu side chain constitutes the immunodominant group. Nevertheless, the tyrosyl residue also contribute considerably to the binding, probably via a peculiar conformation effect, although we can not exclude the tyrosyl residue acting as a secondary contact residue. This monoclonal antibody has been used in a radioimmunoassay to determine leu5 enkephalin like immunoreactive material present in rat brain and hypothalamus. © 1986. |
