par Nijs, Michelle 
;Coune, André ;Tagnon, Henri
Référence Journal of receptor research, 9, 3, page (271-295)
Publication Publié, 1989
;Coune, André ;Tagnon, Henri Référence Journal of receptor research, 9, 3, page (271-295)
Publication Publié, 1989
Article révisé par les pairs
| Résumé : | In order to delineate optimal conditions for the determination of androgen specific binding in human benign prostatic hypertrophy tissues (BPH), extracts of these tissues were prepared using buffers of different compositions. The binding of 5α-dihydrotestosterone (DHT) and of methyltrienolone (R 1881) was analyzed using agar gel electrophoresis. This method allowed the detection of 3 different high affinity tissular binding peaks with similar specificity. Moreover, the inhibition by each of the competitors was also the same for both ligands. It could be demonstrated that none of the observed peaks resulted from the binding of 1 of the ligands to sex hormone binding globulin (SHBG) or to a progesterone receptor. Hypotheses about the possible origin of this peaks are discussed. |
