par Malaisse, Willy ;Zhang, Y.;Louchami, Karim ;Sharma, Sandhya ;Dresselaers, Tom;Himmelreich, U;Novotny, G.W.;Mandrup-Poulsen, T.;Waschke, D;Leshch, Y;Thimm, J;Thiem, J;Sener, Abdullah
Référence Archives of biochemistry and biophysics, 517, page (138-143)
Publication Publié, 2012
Référence Archives of biochemistry and biophysics, 517, page (138-143)
Publication Publié, 2012
Article révisé par les pairs
Résumé : | Suitable analogs of d-mannoheptulose are currently considered as possible tools for the non-invasive imaging of pancreatic islet insulin-producing cells. Here, we examined whether 19F-heptuloses could be used for non-invasive imaging of GLUT2-expressing cells. After 20 min incubation, the uptake of 19F-heptuloses (25 mM) by rat hepatocytes, as assessed by 19F NMR spectroscopy, ranged from 0.50 (1-deoxy-1-fluoro-d- mannoheptulose and 3-deoxy-3-fluoro-d-mannoheptulose) to 0.25 (1,3-dideoxy-1,3-difluoro-d-mannoheptulose) and 0.13 (1-deoxy-1-fluoro-d- glucoheptulose, 3-deoxy-3-fluoro-d-glucoheptulose and 1,3-dideoxy-1,3-difluoro- d-glucoheptulose) μmol per 3 × 10 6 cells. 19F MRI experiments also allowed the detection of 1-deoxy-1-fluoro-d-mannoheptulose in rat hepatocytes. All three 19F-mannoheptuloses cited above, as well as 7-deoxy-7-fluoro-d-mannoheptulose and 1-deoxy-1-fluoro-d-glucoheptulose inhibited insulin release evoked in rat isolated pancreatic islets by 10 mM d-glucose to the same extent as that observed with an equivalent concentration (10 mM) of d-mannoheptulose, while 3-deoxy-3-fluoro-d-glucoheptulose and 1,3-dideoxy-1,3-difluoro-d-glucoheptulose (also 10 mM) were less potent than d-mannoheptulose in inhibiting insulin release. The 1-deoxy-1-fluoro-d- mannoheptulose and 3-deoxy-3-fluoro-d-mannoheptulose only marginally affected INS-1 cell viability. These findings are compatible with the view that selected 19F-heptuloses may represent suitable tools for the non-invasive imaging of hepatocytes and insulin-producing cells by 19F MRI. © 2011 Elsevier Inc. All rights reserved. |