par Welsh, M;Sener, Abdullah 
;Malaisse Lagae, Francine ;Malaisse, Willy
Référence Molecular and cellular biochemistry, 63, page (33-37)
Publication Publié, 1984
;Malaisse Lagae, Francine ;Malaisse, Willy Référence Molecular and cellular biochemistry, 63, page (33-37)
Publication Publié, 1984
Article révisé par les pairs
| Résumé : | Aminooxyacetate, an inhibitor of cytosolic transamination reactions, inhibited insulin release evoked by either 2-ketoisocaproate or L-leucine in rat pancreatic islets incubated in the presence of L-glutamine or L-asparagine. As a rule, aminooxyacetate also inhibited the oxidation of these nutrient secretagogues and impaired the respiratory response of the islets to the combinations of nutrients. However, the oxidative and secretory response to the combination of L-leucine and L-glutamine was less severely affected by aminooxyacetate than that evoked by the three other combinations of exogenous nutrients. These findings reinforce the view that the stimulus-secretion coupling of insulin release in response to L-leucine and 2-ketoisocaproate in association with either L-glutamine or L-asparagine tightly depends on the oxidation of these nutrient secretagogues, on their effect upon O2 uptake and, within limits, on the intracellular site of generation of reducing equivalents in the pancreatic islet cells. © 1984 Martinus Nijhoff Publishers. |
