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Differential IFN-g production by adult and neonatal blood CD56(+) NK and NK-like-T cells in response to T. cruzi and IL-15.

par Guilmot, Aline ;Carlier, Yves ;Truyens, Carine
Référence Parasite immunology, 36, 1, page (43-52)
Publication Publié, 2013-09

Article révisé par les pairs

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Résumé :

Early interferon-gamma (IFN-γ) release by innate cells is critical to direct type 1 immune response able to control intracellular pathogens like Trypanosoma cruzi. Though CD56(bright) Natural Killer (NK) cells are reported to be potent early IFN-γ producers, other CD56(+) cells like CD56(dim) NK cells and NK-like T cells have recently been shown to also release IFN-γ. We have here studied the contribution of each CD56(+) lymphocyte populations in early IFN-γ production in both adults and neonates. On this purpose, we analyzed the kinetics of IFN-γ production by RT-PCR, ELISA and flow cytometry from 2h onwards after T. cruzi and IL-15 stimulation and sought for the responding CD56(+) cells. CD56(bright) and CD56(dim) CD16(-) NK cells were the more potent IFN-γ early producers in response to IL-15 and parasites in adults and neonates. In both age groups, the majority of IFN-γ producing cells were NK cells. However, on the contrary to neonates, CD3(+) CD56(+) NK-like T cells and CD3(+) CD56(-) "classical" T cells also contributed to early IFN-γ production in adults. Altogether, our results support that whereas NK cells responded almost similarly in neonates and adults, cord blood innate CD56(+) and CD56(-) T cells displayed major quantitative and qualitative defects that could contribute to the well-known neonatal immune immaturity. This article is protected by copyright. All rights reserved.