Résumé : The production of essential oil by tissue cultures of Anthemis nobilis L. including cell suspension, shoot and crown-gall cultures is reported. The biosynthetic capability of these different cultures was compared with that of plants grown in a field.The addition of crude polysaccharide fraction prepared from yeast extract and from the plant itself to shoot cultures affected the composition and total essential content (from 0.08 % to 0.30 % dry weight). The essential oil content of the crown-gall tissue of A. nobilis was 0.25 % of the dry weight and the composition of the essential oil was comparable to that of the flowers.